Site Directed Mutagenesis (SDM) Dog Point mutation

Get tips on using Annexin V-FITC Apoptosis Kit to perform Apoptosis assay cell type - SMMC-7721, HEPG2

Get tips on using Annexin V-FITC Apoptosis Kit to perform Apoptosis assay cell type - SMMC-7721, HEPG2

Get tips on using Polybrene Infection / Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines SMMC-7721

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines SMMC-7721

Get tips on using Annexin V-FITC Apoptosis Detection Kit to perform Apoptosis assay cell type - SMMC-7721, HEPG2

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - SMMC-7721, Huh7, Hep3B, 293T

Get tips on using jetPEI® DNA transfection, HTS application to perform DNA transfection Mammalian cells - Immortalized cell lines SMMC-7721

Get tips on using Cell Proliferation Reagent WST-1 to perform Cell cytotoxicity / Proliferation assay cell type - SMMC-7721, Huh7, Hep3B, 293T

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.