PCR Methylation specific PCR

Get tips on using Mastermix 16S Complete to perform PCR Quantitative real-time PCR - Bacterial DNA

Get tips on using HotStarTaq DNA Polymerase (25000) to perform PCR Hot start PCR - Bacterial DNA

Get tips on using QuantiTect Virus Kit (1000) to perform PCR Quantitative real-time PCR - Viral

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Hot start PCR - Bacterial DNA

Get tips on using GoTaq® DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using HotStarTaq Plus DNA Polymerase to perform PCR Conventional / Qualitative PCR - bacterial DNA

Get tips on using TopTaq Master Mix Kit to perform PCR Conventional / Qualitative PCR - mammalian DNA

Get tips on using FastLane Cell Multiplex Kit (200) to perform PCR Multiplex PCR - Mammalian DNA

I would like to preserve leukocytes for future epigenetic analysis. How can I preserve them effectively in order to perform DNA methylation profiling at a later time?

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.