DNA transfection Mammalian cells

Get tips on using HyClone™ Dulbecco's Modified Eagles Medium to perform Mammalian cell culture media MDA-MB-231

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using Gibco™Ham's F-12 Nutrient Mix to perform Mammalian cell culture media PC-3

Get tips on using FGMTM-3 Cardiac Fibroblast Growth Medium-3 BulletKit to perform Mammalian cell culture media HCF

Get tips on using RPMI 1640, with L-Glutamine and 25mM HEPES to perform Mammalian cell culture media DU145

Get tips on using RPMI 1640, with L-Glutamine and 25mM HEPES to perform Mammalian cell culture media VCaP

Get tips on using McCoy's 5A Medium, ATCC® 30-2007™ to perform Mammalian cell culture media A253

Acid phosphatase detection heavily relies on determining the concentration of tartrate-resistant acid phosphatase (TRAP) in the sample. Hence, sample preparation is very crucial and it should be done strictly as per kit manufacturer instructions to avoid any inconsistency and poor sensitivity.

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using EGMTM-2 Endothelial Cell Growth Medium-2 BulletKit to perform Mammalian cell culture media CADMEC/HMVEC