siRNA / RNAi /miRNA transfection Human Cells Cal 27 cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary canine marrow stromal cells

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Anti-Human CD284 (TLR4) to perform Flowcytometry TLR4 (CD284) - Mouse / IgG1, kappa Human Brilliant violet 421

Get tips on using Human Apoptosis Array G1 to perform Apoptosis assay cell type - PC-3

Get tips on using Human MesoEndo Cell Growth Medium to perform Mammalian cell culture media HCAEC

Get tips on using Human MesoEndo Cell Growth Medium to perform Mammalian cell culture media HCtASMC

Get tips on using Human MesoEndo Cell Growth Medium to perform Mammalian cell culture media HCtAEC

Get tips on using Human PLZF Antibody to perform Immunohistochemistry Mouse - PLZF

Get tips on using Human SOX9 Antibody to perform Immunohistochemistry Mouse - SOX9

Get tips on using Human TAK1 Antibody to perform Western blotting Tak1