siRNA / miRNA gene silencing Human Calu-3

Microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using GeneArt™ Site-Directed Mutagenesis PLUS System to perform Site Directed Mutagenesis (SDM) Mouse - Point mutation L929 SigmaR1 gene (σ1)

Get tips on using Nitrocef disks to perform Reporter gene assay β-lactamase substrates - HEK 293 & HEK 293T cells

Get tips on using Beta-Galactosidase Assay to perform Reporter gene assay β-galactosidase substrates - INS-1 832/13

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - HEK 293T RBP3

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Caco-1 CHST11

Get tips on using Senescence Detection Kit - Biovision to perform Reporter gene assay β-galactosidase substrates - mouse cardiac fibroblasts

Get tips on using Senescence Detection Kit - Biovision to perform Reporter gene assay β-galactosidase substrates - mouse embryonic fibroblasts

Get tips on using Senescence Detection Kit - Merck to perform Reporter gene assay β-galactosidase substrates - MDA-MB-231

Get tips on using MethylEasy™ Xceed (ME002) to perform DNA methylation profiling Gene specific profiling - UMR-106 BMP2