Get tips on using lentiCRISPR v2 to perform CRISPR Human - Repression miR-21
Get tips on using EZ-ChIP™ to perform ChIP Human - HEK 293
Get tips on using TTF1 Monoclonal Antibody (2F4D8) to perform Immunohistochemistry Human - TTF-1
Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - PANC-1
Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - MCF-7
Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Human - SH-SY5Y
Get tips on using MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging to perform ROS assay cell type - A549 human adenocarcinomic human alveolar basal epithelial cells
Get tips on using β-Galactosidase Enzyme Assay System with Reporter Lysis Buffer to perform Reporter gene assay β-galactosidase substrates - SK-Hep-1
Get tips on using Hoechst 33258 to perform DNA quantification Human - MDA-MB-231
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
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