Cell cytotoxicity / Proliferation assay

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay HeLa

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay A549

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay U266

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay U266 -

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - L29 mouse fibroblast

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay A-375

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay HCT 116

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay DLD-1

Get tips on using CometAssay Single Cell Gel Electrophoresis Assay to perform DNA Damage Assay Caco-2