Microarray Gene expression arrays

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram negative Helicobacter pylori

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Streptomyces. Sp

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Enterococcus faecium

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Bacteria - Gram positive Bacillus subtilis

Get tips on using Gentra Puregene Mouse Tail Kit (4 g) to perform DNA isolation / purification Tissue - murine tail biopsies

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells Rat cortical neurons

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes

Get tips on using illustra tissue and cells genomicPrep Mini Spin Kit to perform DNA isolation / purification Tissue - kidney

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.