Site Directed Mutagenesis (SDM) Rat Point mutation Rat-2

Get tips on using Sensiscript RT Kit (200) to perform cDNA synthesis Cell lines

Get tips on using ChIP Kit Magnetic - One Step (ab156907) to perform ChIP Human - SMMC-7721

Get tips on using QuantiNova Multiplex RT-PCR Kit (2500) to perform PCR Multiplex PCR - Viral

Get tips on using QuantiNova Probe RT-PCR Kit (2500) to perform PCR Quantitative real-time PCR - Viral

Get tips on using QuantiNova SYBR Green RT-PCR Kit (2500) to perform PCR Quantitative real-time PCR - Viral

Get tips on using QIAGEN OneStep Ahead RT-PCR Kit (200) to perform PCR Quantitative real-time PCR - Fish species DNA

Get tips on using Cell Counting Kit-8 to perform Cell cytotoxicity / Proliferation assay cell type - SMMC-7721, Huh7, Hep3B, 293T

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Get tips on using Cell Proliferation Reagent WST-1 to perform Cell cytotoxicity / Proliferation assay cell type - SMMC-7721, Huh7, Hep3B, 293T

Get tips on using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003 to perform ChIP Human - SMMC-7721