Site Directed Mutagenesis (SDM) Human Point mutation H1299

Get tips on using Absolutely RNA Microprep Kit to perform RNA isolation / purification Cells - immortalized H1299

Get tips on using HyClone RPMI 1640 media: Liquid to perform Mammalian cell culture media H1299

Get tips on using Gibco™ RPMI 1640 Medium to perform Mammalian cell culture media H1299

Get tips on using DMEM - Dulbecco's Modified Eagle Medium to perform Mammalian cell culture media H1299

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - H1299

Get tips on using Luminescent β-galactosidase Detection Kit II to perform Reporter gene assay β-galactosidase substrates - H1299

Get tips on using Senescence β-Galactosidase Staining Kit - Cell Signaling to perform Reporter gene assay β-galactosidase substrates - H1299

Get tips on using CultreCoat BME Cell Invasion Optimization Assay, 96 well to perform Cell migration / Invasion cell type - H1299

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.