protein-expression-and-purification-bacteria-dh10bac-gys1-gn1

Get tips on using CelLytic™ Y Plus Kit to perform Protein isolation Yeast - Saccharomyces cerevisiae

Get tips on using CelLytic™ Y Plus Kit to perform Protein isolation Yeast - Pichia pastoris

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using RIPA Buffer to perform Protein isolation Mammalian cells - SK-N-BE(2)-C

Get tips on using RIPA Buffer (10X) to perform Protein isolation Mammalian cells - Human gingival epithelial cells

Get tips on using IGEPAL® CA-630 to perform Protein isolation Mammalian cells - Human lung fibroblasts

Get tips on using CRP (Human) ELISA Kit (KA0238) to perform ELISA Human - C-Reactive Protein/CRP

Get tips on using QuantiPro™ BCA Assay Kit to perform Protein quantification Mammalian cells - HEK 293

Get tips on using TAGZyme Qcyclase/pGAPase Enzymes (150 U) to perform Protein tag His-tag removal

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Mouse heart