siRNA / miRNA gene silencing Human CAL-27

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human carotid artery endothelial cells

Bacterial culture is a process of letting bacteria multiply in a controlled fashion (temperature, humidity, oxygen content or shaking), in a predetermined culture medium (antibiotic resistance to obtain homogenous clones). It is an important step, especially during cloning, as a single cell can be grown homogeneously (on semi-solid or in liquid conditions) to obtain colonies. As mentioned, bacteria can be cultured in broth cultures (Luria broth or LB) or Petri dishes (Agar plates). A specific antibiotic can be added to the broth or agar plates in order to grow bacteria which have the gene insert conferring its resistance to that antibiotic. Following points are necessary to consider for optimal growth conditions: 1. In general, most bacteria grow well at 37C, but there are some strains which require growth temperatures between 25-30C. 2. It is ideal in broth cultures to fill the flask to ⅓ or less of the total flask volume for optimal aerobic growth. 3. Shaking speeds between 140-180 rpm are appropriate to ensure aeration and that the cells are surrounded by fresh media, and do not settle.

Get tips on using Anti-Glucocorticoid Receptor antibody (ab3578) to perform Immunohistochemistry Human - GR/glucocorticoid receptor

Get tips on using Chromatin Immunoprecipitation (ChIP) Assay Kit to perform ChIP Human - MIA PaCa-2

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Human - MDA-MB-231

Get tips on using ScriptSeq Complete Gold Kit (Epidemiology) to perform RNA sequencing Human - SH-SY5Y

Get tips on using 7-AAD (7-Aminoactinomycin D) to perform DNA quantification Human - PC-3

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - WI-38

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - HEK 293

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - THP 1