dna-isolation-purification-cells-primary-cells-mouse-embryonic-fibroblast-mef

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines HeLa

Get tips on using Lipofectamine® LTX Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines BXPC3

Get tips on using β-Gal Reporter Gene Assay, chemiluminescent to perform Reporter gene assay β-galactosidase substrates - mouse mesenchymal stem cells

Get tips on using Luminescent β-galactosidase Detection Kit II to perform Reporter gene assay β-galactosidase substrates - mouse mesenchymal stem cells

Get tips on using TaqMan® MicroRNA Reverse Transcription Kit to perform siRNA / RNAi /miRNA transfection Mouse - Glomerular Mesangial cells polymer / lipid

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit to perform Live / Dead assay mammalian cells - mouse keratinocytes

Get tips on using ON-TARGETplus Mouse Zbp1 (58203) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Mouse - 3T3-SA Zbp1/Dai

Get tips on using CpGenome Universal DNA Modification Kit to perform DNA methylation profiling Gene specific profiling - MCF-7 Estrogen receptor alpha

Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - Mouse skeletal muscle cells