siRNA / RNAi /miRNA transfection Human Cells THP-1

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human colorectal adenocarcinoma cells (CL-187)

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human peripheral blood monocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human fibroblast-like synoviocytes

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary human chondrocytes - rheumatoid arthritis

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human lung fibroblasts

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human dermal fibroblasts

Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Cells - primary human dermal fibroblasts

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human chondrocytes - osteoarthritis