Get tips on using Purified Mouse Anti-E-Cadherin Clone 36/E-Cadherin (RUO) to perform Immunohistochemistry Human - E-Cadherin
Get tips on using SCGB1A1 antibody (Secretoglobin, Family 1A, Member 1 (Uteroglobin)) (Middle Region) to perform Immunohistochemistry Human - SCGB1A1 /CC10
Get tips on using Monoclonal Mouse Anti-Thyroid Transcription Factor (Concentrate) Clone 8G7G3/1 to perform Immunohistochemistry Human - TTF-1
Get tips on using Purified Mouse Anti-β-Catenin Clone 14/Beta-Catenin (RUO) to perform Immunohistochemistry Human - β-catenin
Get tips on using SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004 to perform ChIP Human - PANC-1
Get tips on using Corning® BioCoat™ Angiogenesis System: Endothelial Cell Tube Formation to perform Angiogenesis assay human - HMVEC
Get tips on using Cultrex® In Vitro Angiogenesis Assay Endothelial Cell Invasion Kit to perform Angiogenesis assay human - HMVEC
Get tips on using MEBMTM Mammary Epithelial Cell Growth Basal Medium to perform 3D Cell Culture Media Human primary breast ephitelial cells-Mammospheres
When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells to perform Live / Dead assay mammalian cells - human fibroblast tissue
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