shRNA gene silencing Mouse Prostate cancer cell lines (DU145 and PC3) CD24

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using Cell Proliferation Kit (XTT based) to perform Cell cytotoxicity / Proliferation assay cell type - HCT116

Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - HT-29

Get tips on using Cultrex® Cell Migration Assay to perform Cell migration / Invasion cell type - MHCC-97H

Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - SH-SY5Y

Get tips on using Cell Proliferation Kit I (MTT) to perform Cell cytotoxicity / Proliferation assay cell type - A549

Get tips on using Cell Proliferation Kit I (MTT) to perform Cell cytotoxicity / Proliferation assay cell type - HeLa

Get tips on using CD74 Antibody (LN-2): sc-6262 to perform Flow cytometry Anti-bodies Mouse - CD74

Get tips on using CD34 Monoclonal Antibody (RAM34), eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD34

Get tips on using ORIS™ Cell Migration Assay to perform Cell migration / Invasion cell type - MDA-MB-231