RNA isolation / purification Tissue Human

Get tips on using FlashTag™ Biotin HSR RNA Labeling Kits to perform Microarray RNA amplification & Labeling - Rat saphenous arteries Biotin

Get tips on using Human IL-1 beta/IL-1F2 Quantikine ELISA Kit to perform ELISA Human - IL-1 beta

Get tips on using Human ICAM-1/CD54 Allele-specific Quantikine ELISA Kit to perform ELISA Human - ICAM-1/CD54

Get tips on using Human/Mouse/Rat Activin A Quantikine ELISA Kit to perform ELISA Rat - Activin

Get tips on using NLRP3/NALP3 (human) monoclonal antibody (Nalpy3-b) to perform Western blotting NLRP3

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Hello everyone! I am currently using different DNA isolation kits to extract DNA from insects. Even though I am able to successfully extract DNA I would like to maximize the yield. Do you have any tips that might help me with that even if the kits are not specifically designed for insect samples?

Get tips on using PE anti-human CD135 (Flt-3/Flk-2) Antibody to perform Flow cytometry Anti-bodies Human - CD135

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.

Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.