Immunohistochemistry 53BP2 phospho (ser-25) Rabbit IgG

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Get tips on using RevertAid First Strand cDNA Synthesis Kit to perform cDNA synthesis Bacteria

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Get tips on using peSpCas9(1.1)-2×sgRNA (empty, donor) to perform CRISPR Human - Repression SLC7A11

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Get tips on using IRDye® 800CW Streptavidin to perform Western blot Biotin - -NA- -NA- Infrared dye 800CW

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A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Apoptosis assay cell type Human endometrial stromal cells

RNA siRNA / miRNA gene silencing Rat Brain endothelial cells HIF-1α Lipid

Get tips on using Ni-NTA HisSorb Strips (24) to perform Protein tag Detection of His-tagged proteins

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Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - mouse MS1

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Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - human BEAS2B

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Get tips on using Cell Comb™ Scratch Assay to perform Wound healing assay cell type - human A549

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Get tips on using CM-H2DCFDA (General Oxidative Stress Indicator) to perform ROS assay cell type - PC12

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