Site Directed Mutagenesis (SDM) Human Point mutation Huh7

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.

Get tips on using Apoptosis/ Necrosis Assay Kit (blue, green, red) (ab176749) to perform Necrosis HuH-7

Get tips on using CardioTACS™ In Situ Apoptosis Detection Kit to perform Apoptosis assay cell type - Cardiomyocytes

Get tips on using In Situ Cell Proliferation Kit, FLUOS to perform Cell cytotoxicity / Proliferation assay cell type - HUVEC

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform Apoptosis assay cell type - HUVEC

Get tips on using VasoTACS™ In Situ Apoptosis Detection Kit to perform TUNEL assay cell type - Mouse endothelial cells

Get tips on using ApopTag® Peroxidase In Situ Apoptosis Detection Kit to perform Apoptosis assay cell type - HEK293

Get tips on using TumorTACS™ In Situ Apoptosis Detection Kit to perform TUNEL assay cell type - Mouse liver tissue

Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - Mouse liver tissue

Get tips on using TdT In Situ Apoptosis Detection Kit - Fluorescein to perform TUNEL assay cell type - Rat fibroblast-like synoviocytes