siRNA / miRNA gene silencing Human 501 Mel and SK Mel 28 FANCD2

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media human umbilical mesenchymal stem cells (hUMSCs) differentiation into osteogenic cells

Get tips on using EpiTect Bisulfite Kit to perform DNA methylation profiling Gene specific profiling - HepG2 Fibrinogen

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell Differentiation media human umbilical mesenchymal stem cells (hUMSCs) differentiation into osteogenic cells

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - HEK 293T RBP3

Get tips on using EZ DNA Methylation kit to perform DNA methylation profiling Gene specific profiling - Caco-1 CHST11

Get tips on using EZ DNA Methylation-Direct Kit to perform DNA methylation profiling Gene specific profiling - A54 RASSF1A

Get tips on using EZ DNA Methylation-Gold Kit to perform DNA methylation profiling Gene specific profiling - SiHa TIMP2

Get tips on using EZ DNA Methylation-Gold Kit to perform DNA methylation profiling Gene specific profiling - HeLa TIMP2

Get tips on using Senescence Detection Kit - Merck to perform Reporter gene assay β-galactosidase substrates - MDA-MB-231