Cell cycle assay human

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Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform 3D Cell Culture Media Primary human breast tumors-Mammospheres

Products Sigma-Aldrich Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Stem cell culture media Brain organoids from Human iPSCs

Products Sigma-Aldrich Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.

DNA DNA transfection Mammalian cells Primary cells Rat pulmonary artery smooth muscle cell (pPASMC)

Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell Differentiation media human umbilical mesenchymal stem cells (hUMSCs) differentiation into osteogenic cells

Products Thermo Fisher Scientific Gibco™DMEM, low glucose, pyruvate

Get tips on using AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit to perform Cell line authentication Human iPSC cells derived from peripheral blood mononuclear cells

Products Thermo Fisher Scientific AmpFLSTR™ Identifiler™ Plus PCR Amplification Kit

DNA DNA isolation / purification Cells Immortalized cell lines H1 hESc

Cell culture media 3D Cell Culture Media Mouse primary mammary ephitelial cells- organoids

Cell culture media 3D Cell Culture Media Mouse primary lung epithelial cells-organoids

Cell culture media 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

Get tips on using Gibco™ MEM α, GlutaMAX™ Supplement, no nucleosides to perform Stem cell Differentiation media Human oogonial stem cells differentiation into oocytes

Products Thermo Fisher Scientific Gibco™ MEM α, GlutaMAX™ Supplement, no nucleosides

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