Protein expression and purification Yeast

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Get tips on using E.Z.N.A.® Yeast RNA Kit to perform RNA isolation / purification Yeast - Schizosaccharomyces pombe

Products Omega Bio Tek E.Z.N.A.® Yeast RNA Kit

Get tips on using Gentra Puregene Yeast/Bact. Kit to perform DNA isolation / purification Yeast - Candida parapsilosis

Products Qiagen Gentra Puregene Yeast/Bact. Kit

Get tips on using Gentra Puregene Yeast/Bact. Kit to perform DNA isolation / purification Yeast - Candida albicans

Products Qiagen Gentra Puregene Yeast/Bact. Kit

Get tips on using pET-21a(+) DNA to perform Protein expression and purification Bacteria - Escherichia coli Prefoldin (PFD)

Products Merck Millipore pET-21a(+) DNA

Get tips on using pQE-80L vector to perform Protein expression and purification Bacteria - Escherichia coli IFABP-Aβ

Products Creative Biogene pQE-80L vector

Get tips on using pGEX-6P-1 Vector to perform Protein expression and purification Bacteria - Escherichia coli FleN

Products Sigma-Aldrich pGEX-6P-1 Vector

Get tips on using pQE-30 Xa Vector to perform Protein expression and purification Bacteria - Escherichia coli Siva1

Products Qiagen pQE-30 Xa Vector

I am currently using a recombinant protein which shows metal-dependent DNase activity. Is it possible to pinpoint the source of the DNase activity after protein purification? More specifically, can I ensure that the DNase activity is not because of nuclease contamination from the E.coli that might have persisted and passed with the protein of interest during purification?

Discussions Is a bacterial nuclease contamination possible during protein purification?

Get tips on using Freedom™ DG44 Kit to perform Protein expression and purification Mammalian cells - CHO-K1 G12

Products Thermo Fisher Scientific Freedom™ DG44 Kit

Get tips on using FastRNA™ SPIN Kit for Yeast to perform RNA isolation / purification Yeast - Pichia pastoris

Products MP Bio FastRNA™ SPIN Kit for Yeast

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