siRNA / miRNA gene silencing Mouse mESC

Get tips on using Live/Dead Double Staining Kit (Merck) to perform Live / Dead assay bacteria - Fusobacterium nucleatum

Get tips on using Live/Dead Double Staining Kit (Merck) to perform Live / Dead assay bacteria - Pseudomonas aeruginosa

Get tips on using Live/Dead Double Staining Kit (Merck) to perform Live / Dead assay bacteria - Porphyromonas gingivalis

Get tips on using miScript SYBR Green PCR Kit (2000) to perform PCR Quantitative real-time PCR - Mammalian DNA

Get tips on using Live/Dead Double Staining Kit (Merck) to perform Live / Dead assay mammalian cells - THP-1

Get tips on using SmGMTM- 2 Smooth Muscle Cell Growth Medium -2 BulletKitTM to perform Mammalian cell culture media HCASMC

Get tips on using SmGMTM- 2 Smooth Muscle Cell Growth Medium -2 BulletKitTM to perform Mammalian cell culture media HCtASMC

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.