siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells

Get tips on using LC3B (D11) XP® Rabbit mAb to perform Autophagy assay cell type - Human fetal osteoblastic (hFOB) 1.19

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Human fetal osteoblastic (hFOB) 1.19

Get tips on using TiterTACS™ Colorimetric Apoptosis Detection Kit to perform TUNEL assay cell type - PC-3 human prostate cancer

Get tips on using DMEM:F12 with HEPES, Glucose and L-Glutamine to perform 3D Cell Culture Media Human lung cancer organoids

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human RT-7

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human BxPC-3

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human epidermal keratinocytes

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human Caco-2

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human MCF-7

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.