Get tips on using RNAqueous®-Micro Total RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MDA-MB-361
Get tips on using mTOR Antibody #2972 to perform Autophagy assay cell type - SH-SY5Y
Get tips on using Medium 199 to perform Mammalian cell culture media PAOSMC
Get tips on using Human Melanocyte Media to perform Mammalian cell culture media HEM
Get tips on using Medium 254CF, Kit to perform Mammalian cell culture media HEM
Get tips on using RPMI 1640, 1X to perform Mammalian cell culture media HepG2
Get tips on using Medium 231 to perform Mammalian cell culture media HAOSMC
Get tips on using Medium 231 to perform Mammalian cell culture media BPAEC
Get tips on using Medium 231 to perform Mammalian cell culture media DAOY
A key signature for necrotic cells is the permeabilization of the plasma membrane. Necrosis can be quantified by several cellular and biochemical assays. When studied minutely, it reveals the difficulty in confirmation in secondary induction of necrosis in apoptotic cells. Apoptotic cells are being analyzed to shift to necrotic status owing to membrane permeability at later stages, and thus, discrimination of two cell death becomes critical. Therefore, it is crucial to use a necrosis detection kit or a defined procedure to analyze this unprogrammed form of death in response to immense chemical and physical insults.
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