Protein expression and purification Mammalian cells HeLa

Get tips on using Medium 231 to perform Mammalian cell culture media HAOSMC

Get tips on using Medium 200 to perform Mammalian cell culture media HAOECv

Get tips on using Medium 231 to perform Mammalian cell culture media BPAEC

Get tips on using RPMI 1640 to perform Mammalian cell culture media HepG2

Get tips on using Keratinocyte-SFM to perform Mammalian cell culture media HaCaT

Get tips on using Medium 231 to perform Mammalian cell culture media DAOY

Get tips on using RPMI 1640 to perform Mammalian cell culture media A2780

Get tips on using RPMI 1640 to perform Mammalian cell culture media CHO

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Bacteria - Gram negative Helicobacter pylori

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.