siRNA / miRNA gene silencing Rat MTLn3 (rat mammary adenocarcinoma breast cancer cell line)

Get tips on using Mammalian beta-Galactosidase Assay Kit to perform Reporter gene assay β-galactosidase substrates - Bxpc-3

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - immortalized BEAS-2B

Get tips on using EZ DNA Methylation-Direct Kit to perform DNA methylation profiling Gene specific profiling - A54 RASSF1A

Get tips on using DNeasy Blood & Tissue Kit to perform DNA isolation / purification Cells - Immortalized cell lines C2C12

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines 3T3

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines HeLa

Get tips on using QIAamp DNA Mini Kit to perform DNA isolation / purification Cells - Immortalized cell lines Renal cortical tubule epithelial cells

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Get tips on using EpiTect Fast 96 Bisulfite Kit to perform Bisulfite DNA Modification Cell lines / primary cells