DNA isolation / purification Cells Immortalized cell lines

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay yeast - Clostridium sporogenes

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay yeast - Candida albicans

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay bacteria - Clostridium sporogenes

Get tips on using CultreCoat Medium BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - HCT116

Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - HN4

Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - SCC2

Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - SCC104

Get tips on using CultreCoat Low BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - SCC6