An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using H2A.X Phosphorylation Assay Kit (Flow Cytometry) to perform DNA Damage Assay Human Skin Fibroblast Cell (FSK)
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Get tips on using WST-1 Cell Proliferation Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - MG-63
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Get tips on using CyQUANT® Cell Proliferation Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - Saos-2
Get tips on using MTS Cell Proliferation Colorimetric Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - Panc-1
Get tips on using CyQUANT® Cell Proliferation Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - DU-145
Get tips on using Live and Dead Cell Assay (Abcam) to perform Live / Dead assay mammalian cells - L29 mouse fibroblast
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