Site Directed Mutagenesis (SDM) Mouse 3T3-L1

Get tips on using ON-TARGETplus Mouse Gpam siRNA to perform siRNA / miRNA gene silencing Mouse - Embryonic stem cells Gpat1

Get tips on using Mouse Reg1 Antibody to perform Immunohistochemistry Mouse - Reg1

Get tips on using Mouse Prolactin ELISA to perform ELISA Mouse - PRL

Get tips on using Mouse Adiponectin ELISA to perform ELISA Mouse - Adiponectin

Get tips on using Mouse ANGPTL3 ELISA to perform ELISA Mouse - Angiopoietin-Like 3 (AngptL3)

Get tips on using Donkey anti-mouse IgG to perform Immunohistochemistry Anti-mouse IgG - Donkey Mouse Rhodamin red

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized NIH-3T3

Get tips on using Individual: TRC Mouse Cdh1 shRNA to perform shRNA gene silencing Mouse - 4T1 Cdh1

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.