protein-expression-and-purification-mammalian-cells-hek-293-mt-pa

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 Ham to perform Mammalian cell culture media PANC-1

Get tips on using GeneChip™ Rat Genome 230 2.0 Array to perform Microarray Gene expression arrays - Rat mesothelium Satin cocktail

Get tips on using Rat GE 4x44K v3 Microarray Kit to perform Microarray Gene expression arrays - Rat pancreas tissue Cyanine 3 & cyanine 5

which is a good monoclonal anibody for this protein?

Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?

Get tips on using Smooth Muscle Cell Growth Medium 2 to perform Mammalian cell culture media HCASMC

Get tips on using Canine Smooth Muscle Cell Growth Medium to perform Mammalian cell culture media CnAOSMC

Get tips on using Bovine Brain Endothelial Cell Growth Medium to perform Mammalian cell culture media BBMVEC