The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads can be a best alternative.
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - immortalized A549
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Mouse Testes
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Mouse Spleen
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Mouse Prostate
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Mouse Muscle
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Mouse Eye
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Mouse Cerebellum
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Uterus
Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Tissue - Human Hypothalamus
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