Protein isolation Tissue

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Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - Rat Kidney

Products Roche Lifesciences High Pure FFPET RNA Isolation Kit

Get tips on using PureLink™ FFPE RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Kidney

Products Thermo Fisher Scientific PureLink™ FFPE RNA Isolation Kit

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Kidney

Products Roche Lifesciences High Pure FFPET RNA Isolation Kit

Get tips on using High Pure FFPET RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Breast

Products Roche Lifesciences High Pure FFPET RNA Isolation Kit

Get tips on using RNAqueous™ Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Mouse Uterus

Products Thermo Fisher Scientific RNAqueous™ Total RNA Isolation Kit

Get tips on using MagNA Pure Compact RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Placenta

Products Roche Lifesciences MagNA Pure Compact RNA Isolation Kit

Get tips on using MagNA Pure Compact RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Bronchi

Products Roche Lifesciences MagNA Pure Compact RNA Isolation Kit

Get tips on using EZ-RNA Total RNA Isolation Kit to perform RNA isolation / purification Tissue - Human Brain

Products Biological Industries EZ-RNA Total RNA Isolation Kit

Get tips on using NucleoSpin® RNA/Protein to perform Protein isolation Mammalian cells - Human eutopic endometrial stromal cells

Products Macherey Nagel NucleoSpin® RNA/Protein

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Cellular assays TUNEL assay cell type Mouse mammary gland tissue

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