DNA transfection Mammalian cells

Get tips on using High Pure RNA Isolation Kit to perform RNA isolation / purification Cells - primary human melanocytes

Get tips on using Cytoplasmic and Nuclear RNA Purification Kit to perform RNA isolation / purification Cells - immortalized Akata

Get tips on using SV 96 Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized T84

Get tips on using Cytoplasmic and Nuclear RNA Purification Kit to perform RNA isolation / purification Cells - immortalized JY

Get tips on using illustra™ RNAspin Mini Isolation Kit to perform RNA isolation / purification Cells - immortalized DH82

Get tips on using illustra™ RNAspin Mini Isolation Kit to perform RNA isolation / purification Cells - immortalized Vero

Get tips on using Quant-iT™ RNA Assay Kit to perform RNA quantification Fuorimetric - mouse glial cells

Get tips on using EZ-Magna ChIP™ G - Chromatin Immunoprecipitation Kit to perform ChIP Human - Kupffer Cells

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.