DNA isolation / purification Bacteria Gram negative

Get tips on using pAC2-dual-dCas9VP48-sgExpression to perform CRISPR Human - Activation SOX2

Get tips on using pAC154-dual-dCas9VP160-sgExpression to perform CRISPR Human - Activation HIV-1 5′ LTR

Western blotting is a widely used technique to size separate proteins from a pool of cell or tissue lysates. The technique has 4 major steps: a) gel electrophoresis, b) blocking and treatment with antigen specific antibody, c) treatment with secondary antibody and finally d) detection and visualization. Though western blotting is a widely used technique, detection of specific proteins depends on several factors, the major ones are antibody concentration, incubation time and washing steps. Key points for obtaining clean blots are: always prepare fresh buffer solutions and optimize antibody concentration. Given the advent of high-throughput protein analysis and a push to limit the use of lab consumables, onestep antibodies are developed which recognise protein of interest and also contain a detection label.

Get tips on using γ Tubulin Antibody (TU-30): sc-51715 to perform Western blotting tubulin gamma

Get tips on using Monoclonal Anti-γ-Tubulin antibody produced in mouse to perform Western blotting tubulin gamma

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - C6

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - MRC5

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - HT1080

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - L929

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - U87MG