Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - primary human coronary artery smooth muscle cells
Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Cells - primary canine coronary artery smooth muscle cells
Get tips on using DC™ Protein Assay Kit I to perform Protein quantification Mammalian cells - Rat mesenteric smooth muscle cells
Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells Rat cortical neurons
Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes
Get tips on using MagNA Pure Compact RNA Isolation Kit to perform RNA isolation / purification Cells - primary human mesenchymal stem cells
Get tips on using illustra tissue and cells genomicPrep Mini Spin Kit to perform DNA isolation / purification Tissue - kidney
Get tips on using TransIT®-LT1 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - Cal 27 cells Polymer / lipid
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.
Fill out your contact details and receive price quotes in your Inbox
Outsource experiment