siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p

Get tips on using miRCURY RNA Isolation Kit to perform RNA isolation / purification Cells - immortalized MC-9

Get tips on using Corning® Hepatocyte Culture Media Kit, 500 mL to perform 3D Cell Culture Media Human prostate organoid

Get tips on using Isolate II RNA Mini Kit to perform RNA isolation / purification Cells - immortalized HCA2

Get tips on using pET-INP-cellulase to perform Protein Expression Prokaryotic cells - E. coli INP-cellulase

Get tips on using Live or Dead™ Cell Viability Assay Kit *Green/Red Dual Fluorescence to perform Live / Dead assay mammalian cells - rat endothelial progenitor cells

Get tips on using DCFDA / H2DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - L-02 human fetal hepatocyte

Get tips on using CellROX™ Deep Red Reagent, for oxidative stress detection to perform ROS assay cell type - PANC-, BxPC-3 human pancreas

Get tips on using pGEX-FIP-nha to perform Protein Expression Prokaryotic cells - E. coli FIP Nectria haematococca

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.

Protein isolation is a technique that involves isolation and/ or purification of protein from cells or tissues via chromatography or electrophoresis. The major challenges in protein isolation include: 1. The concentration of proteins in cells is variable and tends to be small for some intracellular proteins. Unlike nucleic acids, proteins cannot be amplified. 2. Proteins are more unstable than nucleic acids. They are easily denatured under suboptimal temperature, pH or salt concentrations. 3. Finally, no generalized technique/protocol can be applied for protein isolation. Proteins may have different electrostatic (number of positively or negatively charged amino acids) or hydrophobic properties. Therefore, protein purification requires multiple steps depending on their charge (a negatively charged resin/column for positively charged proteins and vice-versa), dissolution (using detergents) and unlike in the case of DNA and RNA, instead of using salts, proteins should be isolated by isoelectric precipitation.