There are a plethora of detection methods of cell cytotoxicity and proliferation by flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.
Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - mouse adipose tissue
Get tips on using AllPrep DNA/RNA/miRNA Universal Kit to perform RNA isolation / purification Tissue - human adipose tissue
Get tips on using Direct-zol RNA Kits to perform RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes
Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Bacteria - Gram positive Streptococcus pyogenes
Get tips on using TRIzol™ LS Reagent to perform RNA isolation / purification Bacteria - Gram positive Listeria monocytogens
Get tips on using TRI Reagent™ Solution to perform RNA isolation / purification Bacteria - Gram negative Salmonella typhi
Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Bacteria - Gram negative Helicobacter pylori
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Bacteria - Gram negative Chlamydia pneumoniae
Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Bacteria - Gram positive Staphylococcus aureus
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