siRNA / miRNA gene silencing Human Lymphoma cell line U937

Get tips on using OCT4-PG1 siRNA to perform siRNA / miRNA gene silencing Human - hES cell line H1 (WA01) OCT4-PG1

Get tips on using SIRT1 siRNA and shRNA Plasmids (h) to perform siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) SIRT1

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using STEAP2 metalloreductase to perform siRNA / miRNA gene silencing Human - PC3 (human prostate cancer cell line) STEAP2