DNA isolation / purification Cells Primary cells

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - THP 1

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Mouse - NIH 3T3

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification Human - Hep G2

Get tips on using EpiTect Fast FFPE Bisulfite Kit (50) to perform Bisulfite DNA Modification Fluids

Get tips on using PicoGreen® dsDNA Quantitation Reagent and Kit to perform DNA quantification Blood

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Human umbilical cord tissue

Get tips on using CelLytic™ MT Cell Lysis Reagent to perform Protein isolation Tissue - Rabbit eye retina/choroids

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.