siRNA / miRNA gene silencing Rat Brain endothelial cells HIF-1α

Get tips on using DeadEnd™ Colorimetric TUNEL System to perform TUNEL assay cell type - HNSCC Detroit 562 human head and neck tumor cells

Get tips on using DMEM/F-12, no phenol red to perform 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres

Get tips on using DMEM/F-12 PLUS Basal Medium to perform 3D Cell Culture Media Human breast cancer MDA-MB-231 cells-Mammospheres

Get tips on using Gibco™Advanced DMEM/F-12 to perform Stem cell Differentiation media hPSCs or iPSCs differentiation into Lung progenitor cells

Get tips on using STEMdiff™ SMADi Neural Induction Kit to perform Stem cell Differentiation media Differentiation of Human iPSC into Human Neuroepithelial cells

Get tips on using FITC Annexin V Apoptosis Detection Kit I (RUO) to perform Apoptosis assay cell type - T-cells Mouse (CD4+ and CD8+)

Plasmid isolation is an important technique in molecular biology or any kind of genetic editing. It involves amplifying plasmids overnight by transforming them into competent bacterial cells. The desired colonies of these bacteria can then be grown in shaker cultures, at appropriate shaking speed, oxygen availability and temperature. These liquid cultures can then be ultracentrifuged to pellet the bacteria, which are then used for plasmid isolation. The bacteria are first resuspended in a buffer, then lysed, neutralized, purified in a column, eluted, precipitated with ethanol and then resuspended. During plasmid isolation, it is important to lyse cells quickly because lysing bacteria for too long may lead to irreversible denaturing of the plasmid. Usually, alkaline lysis is used for isolation because it is a mild treatment. It isolates plasmid DNA and other cell components such as proteins by breaking cells apart with an alkaline solution. Precipitation removes the proteins, and the plasmid DNA recovers with alcohol precipitation. Resuspension and lysis buffers should be mixed thoroughly in order to prevent the DNA from breaking into smaller fragments. This is because broken gDNA can reanneal and remain in the solution, without binding to the column.

Get tips on using PE Annexin V Apoptosis Detection Kit with 7-AAD to perform Apoptosis assay cell type - T-cells Mouse (OT-I)

Get tips on using HyClone Dulbecco's Modified Eagle Medium (DMEM)/ F12 1:1: Liquid to perform 3D Cell Culture Media U87MG cells- glioblastoma spheres

Get tips on using HyClone Dulbecco's Modified Eagle Medium (DMEM) with low glucose: Liquid to perform Stem cell culture media hAdipose derived stem cells