Cell cycle assay mouse

Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - LTEP-a-2 lung adenocarcenoma

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

ROS has a very short half-lives in biological environment as they are influenced by exposure to ambient oxygen. As it is highly reactive and hard to measure care should be taken to ensure the stability of the sample during isolation, preparation, storage, and analysis.

Get tips on using QCM™ Tumor Cell Transendothelial Migration Assay (Colorimetric, 24 Assays) to perform Cell migration / Invasion cell type - SW480

Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 8 μm to perform Cell migration / Invasion cell type - BCPAP

Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 8 μm to perform Cell migration / Invasion cell type - SW480

Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 8 μm to perform Cell migration / Invasion cell type - HUVEC

Get tips on using EZViable™ Calcein AM Cell Viability Assay Kit (Fluorometric) to perform Live / Dead assay mammalian cells - rat brain microvascular endothelial cells

Get tips on using Cultrex 3D Culture BME Cell Invasion Assay, 96 well to perform Cell migration / Invasion cell type - BxPC-3

Get tips on using CytoSelect™ 24-Well Cell Migration Assay, 5 µm to perform Cell migration / Invasion cell type - SaOS-2