Cell cycle can be challenging due to difference introduced by sample handling, timing, and difference within the sample. Downstream instriuments to analyse cell cycle (Multicolor flow cytometry and multicolor imaging) can answer these challenges. Relevant markers can be combined with cell phenotyping markers to look at events within subpopulations of cells.
Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - rat MSC
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse C166
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - mouse 4T1
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human HMEC
Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human HUVEC
Get tips on using Secrete-Pair™ Dual Luminescence Assay Kit to perform Reporter gene assay β-galactosidase substrates - HUVEC
Get tips on using Vybrant™ MTT Cell Proliferation Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - FADU
Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Cell cytotoxicity / Proliferation assay cell type - FADU
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