rna-isolation-purification-cells-primary-human-carotid-artery-endothelial-cells

Get tips on using TRI Reagent® Sigma to perform RNA isolation / purification Cells - primary human cardiac fibroblasts

Get tips on using Gentra Puregene Cell Kit to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary porcine primary airway epithelial cell

Get tips on using RNeasy Plus Mini Kit to perform RNA isolation / purification Cells - primary human peripheral blood monocytes

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - primary rat dermal fibroblasts

Get tips on using NucleoBond® RNA/DNA to perform DNA isolation / purification Cells - Primary cells Rat cortical neurons

Get tips on using Pierce™ Cell Surface Protein Isolation Kit to perform Protein isolation Mammalian cells - Human aortic endothelial cells

Isolating DNA from tissues and paraffin-embedded tissue samples can be challenging as double-stranded DNA is physically fragile and highly susceptible to exo- and endonucleases. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in the presence of DNAse inhibitors. Further, extracting DNA from the nucleus need specific methods by combining physical, mechanical and chemical lysis approaches,

Get tips on using NucleoSpin® RNA to perform RNA isolation / purification Cells - primary mouse cortical neurons

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Cells - primary rat aortic smooth muscle cells