Site Directed Mutagenesis (SDM) Human Point mutation MDA-MB-231

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Get tips on using QIAamp Fast DNA Stool Mini Kit (50) to perform DNA isolation / purification Tissue - fecal sample

Products Qiagen QIAamp Fast DNA Stool Mini Kit (50)
EMBacY#1 Product

Get tips on using EMBacY#1 to perform Protein Expression Eukaryotic cells - Hi5 mDicer2

Products Sabine Suppmann, Max-Planck Institute of Biochemistry EMBacY#1

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDCK

Products Thermo Fisher Scientific TRIzol Reagent

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized MDCK

Products Qiagen RNeasy Mini Kit

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Cells - immortalized MDCK

Products Promega SV Total RNA Isolation System

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Discussions How can I improve my DNA yield?

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - Myelodysplastic (MDS‐L)

Products Enzo Life Sciences CYTO-ID® Autophagy detection kit

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase negative control (luciferase vector)

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay β-lactamase substrates HeLa

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cellular assays Reporter gene assay luciferase HepG2 and Huh7 cells

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