siRNA / miRNA gene silencing Human hES cell line H1 (WA01)

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - HT1080

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - L929

Get tips on using Dansylcadaverine to perform Autophagy assay cell type - U87MG

Get tips on using Gibco™DMEM, low glucose, pyruvate to perform Stem cell culture media hMSCs

Get tips on using CYTO-ID® Autophagy detection kit to perform Autophagy assay cell type - HOS

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using William's E Medium, no phenol red to perform 3D Cell Culture Media Mouse liver organoids

Get tips on using Gibco™Advanced DMEM/F-12 to perform 3D Cell Culture Media Mouse liver organoids

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media hTSPCs differentiation into tenogenic cells

Get tips on using Gibco™ DMEM, high glucose to perform Stem cell Differentiation media hTSPCs differentiation into Chondrogenic cells