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Get tips on using In Situ Cell Death Detection Kit, Fluorescein to perform TUNEL assay cell type - 3T3 L1 mouse adipose tissue
Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - BEAS-2B human bronchial epithelial cell line
Get tips on using Click-iT™ EdU Alexa Fluor™ 555 Imaging Kit to perform Cell cytotoxicity / Proliferation assay cell type - PC-3
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The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
Get tips on using Anti-LC3B antibody (ab48394) to perform Autophagy assay cell type - A2058
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