siRNA / miRNA gene silencing Human CRL-5915

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay yeast - Clostridium sporogenes

Get tips on using Viability/Cytotoxicity Assay Kit for Animal Live & Dead Cells to perform Live / Dead assay bacteria - Clostridium sporogenes

Get tips on using Claudin 1 Polyclonal Antibody to perform Western blotting Cclaudin-1

Get tips on using claudin-1 Antibody (XX7): sc-81796 to perform Western blotting Cclaudin-1

Get tips on using SQSTM1 monoclonal antibody (M01), clone 2C11 to perform Autophagy assay cell type - AML12

Get tips on using Recombinant Anti-Claudin 1 antibody [EPR9306] (ab180158) to perform Western blotting Cclaudin-1

Get tips on using Purified Mouse Anti-Beclin Clone 20/Beclin (RUO) to perform Autophagy assay cell type - NIH-3T3

Get tips on using Purified Mouse Anti-Beclin Clone 20/Beclin (RUO) to perform Autophagy assay cell type - THP 1

Get tips on using Purified Mouse Anti-p62 Ick ligand Clone 3/P62 LCK LIGAND (RUO) to perform Autophagy assay cell type - THP 1