siRNA / miRNA gene silencing Rat Cardiomyocyte (H9C2)

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Control siRNA-A to perform siRNA / miRNA gene silencing Human - OV2008 Yap Gene Lipofectamine

Get tips on using YAP siRNA (h) to perform siRNA / miRNA gene silencing Human - OV2008 Yap Gene Lipofectamine

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - H9c2 rat cardiomyocytes

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - H9c2 rat cardiomyocytes

Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - H9c2 rat cardiomyocytes

Get tips on using CD98 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - RAW264.7 CD98

Get tips on using Mm_Hdac5_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 HDAC5

Get tips on using KRAS siRNA to perform siRNA / miRNA gene silencing Human - A431 KRAS

Get tips on using STEAP1 siRNA to perform siRNA / miRNA gene silencing Human - LNCap STEAP1