rna-isolation-purification-cells-immortalized-bxpc-3

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Cell culture media 3D Cell Culture Media Human endometrium organoids

Cell culture media 3D Cell Culture Media Mouse fallopian organoids

Cell culture media 3D Cell Culture Media Mouse endometrium organoids

Cell culture media 3D Cell Culture Media Mouse prostate organoid

Cell culture media 3D Cell Culture Media Human prostate organoid

Cell culture media 3D Cell Culture Media Mouse skin organoids

Cell culture media 3D Cell Culture Media Human gastric organoids

Cell culture media 3D Cell Culture Media Mouse embryonic neurospheres

Cell culture media 3D Cell Culture Media Mouse thymic organoids

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

Discussions siRNA/RNAi/miRNA transfection human

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